Figure 2. Changes in angiotensinogen (AGT) and megalin mRNA expression in unilateral ureteral obstruction (UUO) kidneys of WT, proximal tubule-specific AGT KO (PKO), and liver-specific AGT KO (LKO) mice. WT control, PKO, and LKO mice were subjected to UUO or sham operation for seven days. (A-B) Kidney AGT mRNA levels were measured using quantitative real time RT-PCR. The levels are expressed as a fold increase to Sham (n=5-9). (C-D) Levels of megalin mRNA were measured using quantitative real time RT-PCR. The levels are expressed as a fold increase to Sham in WT control (n=5-7). Data are expressed as means±SD. *p<0.05 vs. WT-Sham; ^#p<0.05 vs. WT-UUO.