Figure 5. Expression of α-SMA and p-Smad3 in unilateral ureteral obstruction (UUO) kidneys of WT, proximal tubule-specific angiotensinogen (AGT) KO (PKO), and liver-specific AGT KO (LKO) mice. (A-C) Levels of α-SMA and p-Smad3 were examined by western blot analysis using specific antibodies. Representative western blots are shown (n=5). Anti-GAPDH antibody was used as a loading control. Expression levels were evaluated using Image J software. S: Sham; Con: control. Data are expressed as means±SD. *p<0.05 vs. WT-Sham.
