Figure 1.

Cryo-EM of AaLS. (a) Representative electron micrograph of AaLS particles taken at an approximate defocus of −1.0 µm and an approximate total fluence of 46 e⁻ Å⁻². (b) Corresponding CTF parameter estimation power spectrum. (c) Summed power spectrum of five micrographs of UltrAuFoil support film indicating the (002) diffraction ring (red circle) used for pixel calibration. The (113) ring is also visible, indicating frequencies to 1.23 Å using conditions identical to data collection. (d) Class averages of 4× binned AaLS particles. Scale bars correspond to 200 Å.