Table 1. Cryo-EM data collection parameters.

Microscope	TFS Titan Krios G4
Detector/energy filter	TFS Falcon 4i/Selectris-X
Magnification (nominal)	270000×
Energy filter slit width (eV)	10
Flux over vacuum (e− pixel−1 s−1)	3.1
Total fluence per movie (e− Å−2)	46
Exposure time (s)	3
EER frames per movie	918
Defocus range (µm) (nominal)	0.4–1.2
Calibrated pixel size (Å)	0.4553
Total movies collected	12657
Average collection speed per hour	315