Figure 1.

Analysis of tRNA-derived sncRNAs in human plasma samples

(A) Schematic representation of the sncRNA sequencing for T4 PNK-treated RNAs.

(B) PCA of tRNA-mapped reads.

(C) Classification of tRNA-derived sncRNAs in this study.

(D) Proportion of tRNA-derived sncRNAs classified to each subclass.

(E) Spearman correlation analysis among each class of tRNA-derived sncRNAs. The color and size of each dot indicate the correlation coefficient calculated as z value.

(F) Proportion of 5′-halves derived from respective cytoplasmic tRNA isoacceptors.

(G) Heatmap and dendrogram showing the clustering of the 5′-half expression pattern.

(H) The levels of 5′-GlyGCC and 5′-ValCAC in plasma samples quantified by TaqMan qPCR. The value of one of the healthy samples was set as 1, and the relative values for the other samples are shown. The sample size in the first set of experiment: Healthy: n = 5; COPD: n = 7. For 5′-GlyGCC, only four healthy samples and six COPD samples were detected and included in the graph. The second set of experiment: Healthy: n = 5; COPD: n = 8. For 5′-GlyGCC, only four healthy samples and three COPD samples were detected. ∗p < 0.05.