Fig. 4.

The relationship between cell types and the expression of MCP-1/CCL2 in scRNA-seq analysis. (a) In tSNE plot of scRNA-seq analysis, the cells were divided into eleven clusters, including epithelial cells (red line marked) and macrophage (blue line marked). (b) Using KRT18 as the epithelial marker gene, the epithelial cells are further divided into seven clusters (C0 to C6). (c) Senescence associated genes of CDKN2A and CDKN2B are mainly expressed in the C3 cluster. (d) Among C0 to C6 clusters, the proportion of MCP-1/CCL2 expressing cells is the highest (7.11 %) in the C3 cluster. (e) In GSEA analysis, up-regulated genes of C3 are enriched in pathways of reactive oxygen species，mTORC1 signaling，IL6-JAK-STAT3 signaling，and so on. (f) Using CSF1R as the marker gene, the macrophages are further divided into six clusters (C0 to C5). (g) In violin plot, the M1 polarization cells (C4) express high levels of CD80 and CD86, while the M2 polarization cells (C0) express high levels of CD206 and CD163. (h) The gene expression feature of MCP-1/CCL2 in macrophages. (i) Among six clusters of macrophages, the proportion of MCP-1/CCL2 expressing cell is the highest (17.64 %) in the C0 cluster. (j) By GSEA analysis, the up-regulated genes of C0/M2 cluster are enriched in pathways of TNFα signaling via NF-κB，inflammatory response，complement，and so on.***P < 0.001.