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. 2024 Sep 3;134(17):e178711. doi: 10.1172/JCI178711

Figure 2. Group 1 ILCs are specifically recruited to sarcoid granulomas.

Figure 2

(A) Gene expression profile of single-cell-identified ILC cluster best matched the ILC definition in the Immunological Genome Project (ImmGen) database. (B) Dot plot comparing gene expression profiles of sarcoidosis immune cells and ILCs to published purified ILC subpopulations. Sarcoidosis ILCs most closely matched ILC1. Dot size reflects percentage cells expressing the gene, and color illustrates level of gene expression. (C) Flow cytometry gating scheme for human ILCs, including NK cells. (D) Flow cytometry analysis of ILC subtypes as percentage of total sorted cells in affected and unaffected sarcoidosis and non-sarcoidosis granuloma skin (n = 3). One-way ANOVA revealed statistical significance for ILC1. *P < 0.05. (E) Comparison of gene expression from flow cytometry–purified sarcoidosis skin ILC1s (n = 3) to sarcoidosis ILCs identified in our scRNA-seq data sets. Eight out of top 10 genes matched and are listed.