Skip to main content
. 2024 Sep 3;134(17):e178711. doi: 10.1172/JCI178711

Figure 6. ILCs are necessary for noninfectious granuloma formation in mice.

Figure 6

(A) Representative histology (n = 2 patients) demonstrating ILCs (Lineage–IL7R+Tbet+) and recruitment of B cells (CD20+CD23+) and to human lung sarcoid granulomas. Lineage– = CD3–CD16–CD19–CD20–CD56–CD68– (labeled in green). Scale bars: 100 μm and 10 μm (insets). (B) Representative H&E staining depicting granuloma formation in whole lung sections from cadmium nanoparticle–treated WT, Rag2–/–, and Rag2–/– Il2rg–/– (ILC-KO) mice. Inset images show higher magnification. Scale bars: 100 μm. (C) Quantification of lung granulomas (WT, n = 6; Rag2–/–, n = 7; ILC-KO; n = 8). (D) Representative immunofluorescence depicting macrophage (F4/80+) accumulation in lung tissue. Two-tailed, unpaired Student’s t test. Scale bars: 100 μm. The WT macrophages are also shown in Supplemental Figure 10C. (E) Flow cytometry analysis of ILC1 and NK cells as percentage of live CD45+ cells in different mouse genotypes before and after treatment with cadmium nanoparticles (QDOT). WT, n = 6; QDOT-treated, WT, n = 4; Rag2–/–, n = 7; ILC-KO, n = 3. Two-tailed, unpaired Student’s t test, comparing WT mice before and after treatment. Data represented as mean ± SEM. **P < 0.01; ***P < 0.001.