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. 2024 Feb 27;23(9):1292–1299. doi: 10.1038/s41563-024-01811-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, Representative load–strain curves of LIFT hydrogels comprising 0%, 5% or 10% w/v four-arm PEG–maleimide crosslinked in CaCl₂/PEG–dithiol for 20 min, 37 °C, 50 RPM. b, Load at 90% strain of the different hydrogel compositions; n = 4 hydrogels were tested. Statistical analysis was performed by one-way ANOVA with post-hoc Tukey’s multiple comparisons test, n = 4 independent experiments. Data are presented as mean ± standard deviation. c, Images of various compositions of hydrogels before and after 90% strain. Scale bars, 5 mm. d, Load–strain curves of LIFT hydrogels formed in various % v/v mixtures of real porcine gastric fluid (rGF) and water containing CaCl₂/PEG–dithiol. e, Gelation kinetics of LIFT hydrogels immersed in a crosslinker bath comprising CaCl₂/PEG–dithiol at 37 °C, as characterized by rheology. f, Cumulative release of 155 kDa dextran and 20- or 100-nm nanoparticles from alginate and LIFT hydrogels. Hydrogels were incubated in SGF or SIF for the indicated time periods. Shown is the average of n = 3 independent experiments.

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