Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Aug 30;14:20223. doi: 10.1038/s41598-024-69658-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

PMC Copyright notice

Histological photography from decalcified samples. (a,b,c) Stained by Masson’s trichrome. (a) From BDT by ELRs after 90 days. It showed bone edge defect (white line) connected to newly formed bone (white asterisk) surrounded by fibroblastic tissue (black asterisk). Decalcified sample.40X. Scale bar 200 μm. (b) At BDT area shows inside fibroblastic tissue (black asterisk) a newly formed trabeculae (white asterisk) with preliminary bone marrow formation (yellow asterisk). Inside, it was observed new vessels formation (black arrows) near to bone surface. 100X. Scale bar = 100 μm. (c) At high magnification, bone formed tissue shows pagetoid pattern (white asterisk) with its irregular arrangement. Mature (red) and immature (blue) bone tissue with osteoblasts on surface (black arrow), and osteocytes (white arrow) inside. 400 X magnification. Scale bar = 20 μm. (d) Decalcified samples stained by Verhoeff´s elastic stain. Bone defect treated (BDT) shows ossicles (white asterisk) formed around structures like biomaterial debris (gray asterisk). New bone was surrounded by fibroblastic tissue (black asterisk) with many angiogenesis formations containing blue outlined erythrocytes (black arrow). Bone adopted an irregular arrangement resembling pagetoid-like bone (white asterisk). 40X magnification. Scale bar = 100 μm. (e) Higher magnification (black square) from 8.d. New pagetoid-like bone (white asterisk) formed on amorphous areas compatible with remains of ELR´s (gray asterisk), surrounded by angiogenesis (black arrow). Osteoblasts (white arrow) and osteocytes (grey arrow) were observed. 400X magnification. Scale bar = 20 μm.