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. 2024 Aug 30;14:20223. doi: 10.1038/s41598-024-69658-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Microscopic images from undecalcified samples embedding in methyl methacrylate stained with Masson-Goldner’s trichrome. (a) In BDUT was observed new bone formation with several islets (white asterisk), immersed in fibroblastic tissue (green asterisk) at control bone defect edge. However, its thickness was minor compared with preexisting bone (black asterisk). Osteoid tissue areas were found (black arrow) on mineralized bone. 40 X. Scale bar = 200 μm. (b) At BDT bone formation showed on its edge and central zone, like bone islets (white asterisk) covered by osteoid tissue (white arrow); all surrounded by fibroblastic tissue (green asterisk). 40 X. Scale bar = 200 μm. (c) At BDT Bone new formation over BDT central zone surrounded by fibroblastic tissue (green asterisk) and new ossicles (white asterisk) cover by red osteoid thick layer (black arrow). 100 X. Scale bar = 100 μm.