Fig. 2. ofCS-scFvs exhibit cancer-selective reactivity in tissues.

a Immunofluorescence (IF) analysis of paraffin-embedded fixed (FFPE) human tissue TP241b micro-array of healthy/normal adjacent (NAT) and malignant tissues from prostate, lung, breast and colon using 25 nM V5-tagged scFv² (F4, F8, F3, C9) or V5-tagged Fab² (B1) detected with Anti-V5-Alexa647 (red). DAPI was used for nuclear staining (blue). b Panel of malignant (top) and healthy (bottom) FFPE sections of different tissue types stained with C9 or F8 scFv² as described in (a). c IF staining of FFPE lung adenocarcinoma (top) and carcinoma (bottom) sections from LC2085 TMA with C9 or F8 scFv² as described in (a). d Mean pixel intensities of tissue micro-array (TMA) from different cancer types with number of sections (n) indicated for each group, stained as described in (a) comparing healthy/normal to malignant tissues from the pancreas (P < 0.0001), breast (P < 0.0001), skin (C9: P < 0.016; F8: P < 0.017), lung (P < 0.0001) the digestive system (C9: P < 0.0009; F8: P = 0.0054) and colon (C9: P = 0.00112; F8: P = 0.0149); healthy/normal to benign from the digestive system (P < 0.0001); and healthy/normal to metastatic colorectal tissues (C9: P = 0.0059; F8: P = 0.026). Intensities were measured on Fiji software and two-tailed unpaired parametric t-test was applied. e IF staining of mouse embryos stained with scFv² as described in (a). To the right, a zoom-in of the dashed box is shown. Source data are provided as a Source data file.