Fig. 4.

200 nm IVIG-SiMPs induced G1 arrest and apoptosis in TNFα- stimulated HUVECs. (A) IVIG-SiMPs, as well as IgG-SiMPs (80 µg/ml) induce apoptosis in TNFα-stimulated HUVECs after 24 h. treatment, as detected by lactadherin/PI assay. (B) The apoptosis indicated by the increase in the TUNEL-positive (TUNEL⁺) cell population and (C) G1 cell cycle arrest in TNFα-stimulated HUVECs after 24 h. of treatment. The cell cycle analysis was performed by flow cytometry using the APO-BrdU™ TUNEL Assay Kit. Mean ± SEM, n = 3, ** p < 0.01, *** p < 0.001