Fig. 3. Immunopeptidomic profile of class I and class II eluted peptides presented by BLCL 48 h after direct delivery of protein antigen.

Samples were analysed using PEAKS Online using a 5% FDR cut-off. A Relevant eluted peptide numbers, headers in bold. B Mean length distribution of HLA I and HLA II eluted peptides. SD is shown, based on all n = 5 cell lines in A; 9087: black bars, 9004: blue bars. C Representative Gibbs Cluster peptide motif analysis of 9-mers from an HLA class I elution of 9004 with delivery of Nucleoprotein is shown. HLA-A*02:01-derived peptides dominate the HLA class I immunopeptidome, cluster size is 2602 peptides. Analysis using Immunolyser⁵³. Nsp non-structural protein, BLCL B lymphoblastoid cell line, HLA human leukocyte antigen. Source data are provided as a Source Data file.