Figure 1.

Schema of thiol-induced and pH-induced cleavage of two various inteins using the chitin column. A) C-terminal fusion of the target protein to the inteins that auto-cleaved by the addition of a reducing agent. B) N-terminal fusion of the target protein to the inteins that auto-cleaved by a shift in the buffer pH. C) C- and N-terminal fusion of the target protein to the inteins that auto-cleaved by the addition of a reducing agent and a shift in the buffer pH, respectively. TP: target protein, T-i: thiol-induced cleavage, pH-i: pH- induced cleavage, IN: intein, and CBD: Chitin Binding Domain.