Table 1.
Tumor characteristics.
| Est. | 95% CI | Est. | 95% CI | p | Est. | 95% CI | p | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Figure 1 | Constant (Control) (%) | PG/VG (/µM) [range 0–20 µM] | Nicotine (%) [no/yes] | |||||||
| Scratch area covered (%) [Panel C] | 16.85 | (14.79, 18.91) | 0.58 | (0.39, 0.77) | <0.001 | .26 | (-2.48, 3.00) | 0.851 | ||
| –Including interaction -0.096 (-0.493, 0.302), p=0.638 |
16.62 | (14.33, 18.92) | 0.61 | (0.37, 0.86) | <0.001 | 1.05 | (-3.24, 5.33) | 0.632 | ||
| Viable cells x 106 [Panel D] | 5.84 | (5.70, 5.98) | 0.060 | (0.048, 0.071) | <0.001 | -0.56 | (-0.72, -0.39) | <.001 | ||
| –Including interaction -0.039 (-0.057, -0.021), p<.001 |
5.71 | (5.60, 5.84) | 0.076 | (0.064, 0.088) | <0.001 | -0.22 | (-0.42, -0.02) | 0.029 | ||
| Supplementary Figure S3 | PG/VG [no/yes] | Nicotine (/mg/ml) [0, 6, 36 mg/ml] | ||||||||
| Tumor bioluminescence on day 15 (p/s/cm2/sr) | 935 | (409, 1462) | 1232 | (567, 1897) | <0.001 | 13.0 | (-6.3, 32.3) | 0.187 | ||
| Figure 4 | PG/VG [no/yes] | Nicotine [no, yes (36 mg/ml)] | ||||||||
| Tumor bioluminescence on day 14 (p/s/cm2/sr) [Panel C] | 2985 | (2257, 3713) | 1617 | (588, 2646) | 0.002 | 857 | (-172, 1886) | 0.103 | ||
| Metastatic nodules on day 22 (number) [Panel F] | 61 | (48, 74) | 59 | (41, 77) | <0.001 | 86 | (68, 103) | <0.001 | ||
| Lung weight on day 22 (mg) [Panel G] | 946 | (731, 1162) | -76 | (-390, 239) | 0.637 | 400 | (86, 714) | 0.013 | ||
| Figure 5 | ||||||||||
| Lung infiltrating macrophage frequency within CD45+ immune cells (%) [Panel A] | 38.6 | (28.9, 48.2) | 6.1 | (-7.9, 20.2) | 0.392 | 24.5 | (10.4, 38.5) | 0.001 | ||
| CD8+ T cell within CD45+ immune cells (%) [Panel B] | 3.93 | (3.74, 4.12) | -1.70 | (-1.96, -1.43) | <0.001 | -0.53 | (-0.79, -0.27) | <0.001 | ||
| IL-6 in lung macrophages [Panel E] | 442 | (140, 743) | 388 | (-39.1, 814.1) | 0.075 | 2270 | (1844, 2697) | <0.001 | ||
| TNFα in lung macrophages [Panel F] | 0 | (-211, 211) | 100 | (-199, 399) | 0.512 | 4135 | (3586, 4434) | <0.001 | ||
| PD-1 in lung CD8+ cells [Panel I] | 20.1 | (17.8, 22.3) | 62.2 | (59.0, 65.4) | <0.001 | -1.6, | (-4.7, 1.6) | 0.335 | ||
| TNFα in lung CD8+ cells [Panel J] | 964 | (862, 1066) | 40 | (-104, 185) | 0.586 | 2963 | (2814, 3112) | <0.001 | ||
| Figure 6A | PG/VG [no/yes] | Nicotine [no, yes (36 mg/ml)] | ||||||||
| CTLA4 (MFI) | 6473 | (5441, 7504) | 910 | (-549, 2369) | 0.221 | 1882 | (423, 3341) | 0.011 | ||
| PD-1 (MFI) | 2486 | (2069, 2902) | 680 | (90, 1269) | 0.024 | -237 | (-826, 353) | 0.431 | ||
| TIM3 (MFI) | 2 | (-51, 56) | 53 | (-23, 129) | 0.169 | 95 | (19, 171) | 0.015 | ||
| LAG-3 (MFI) | 969 | (907. 1032) | 26 | (-63, 115) | 0.566 | -76 | (-165, 12) | 0.091 | ||
| Figure 6C | ||||||||||
| 4exh markers (%) | 5.2 | (4.3, 6.1) | 5.4 | (4.1, 6.6) | <0.001 | 6.7 | (5.5, 8.0) | <0.001 | ||
Analysis done with Stata 15 mixed, REML option.