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. 2024 Aug 30;15:27. doi: 10.1186/s43008-024-00158-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Dose–response curve of the Cryptococcal Antigen Semi-Quantitative (CrAgSQ) lateral flow assay (LFA) for both T1 and T2 lines, revealing the presence of the hook effect for T1 line after approximately 190 ng/ml. B Quantitative results and regression analysis curve depicting the relationship between cryptococcal antigen (CrAg) concentration and band ratio signal intensity (T1/T2)