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. 2024 Mar 9;14(8):3680–3696. doi: 10.1016/j.apsb.2024.03.010

Figure 6.

Figure 6

In vitro ICD effect and DC maturation stimulation. (A) Schematic illustration of the ICD effect induced by CT26 cells treated with different Epi formulations to stimulate mouse bone marrow DC (BMDC) maturation. Representative CLSM images of (B) surface calreticulin exposure and (C) release of high mobility group box 1 (HMGB1) in CT26 cells after culturing with Epi·HCl or Epi-PD for 24 h. (D) Intracellular and (E) extracellular adenosine triphosphate (ATP) concentrations in CT26 cells after the same treatment as (B) (Data are mean ± SD, n = 4). (F) Flow cytometry analysis and (G) statistical data of mature BMDCs (CD80+CD86+ cells in CD11c+MHC II+ cells) in vitro (Data are mean ± SD, n = 3). ns, not significant, ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 compared with the Epi-P4D-treated group.