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. 2024 Aug 31;15:7584. doi: 10.1038/s41467-024-51862-0

Fig. 1. DKO mice are viable but are smaller, with larger SMGs, and have a reduction in specific 3-O-sulfated tetrasaccharides compared to WT.

Fig. 1

a Data shown are the number of mice of a given genotype for each sex derived from 25 independent litters obtained from heterozygous crosses. Chi-square analysis shows no significant difference from the expected ratios. b Body weight and SMG weight of adult male and female mice normalized to body weight and WT. Error bars are SM. Body weight: n = 65 (WT-male), 51 (DKO-male), 49 (WT-female) and 35 (DKO-female) mice. SMG weight: n = 63 (WT-male), 46 (DKO-male), 47 (WT-female) and 42 (DKO-female). Unpaired two-tailed t-test. ***p < 0.0001, compared to WT. c, d Mass spec analysis of HS disaccharides and tetrasaccharides in adult male SMGs. Analysis of HS disaccharide (c) and tetrasaccharide (d) composition between WT and DKO SMGs. All data shown is averaged and presented as stacked bar graphs. n = 3 biological repeats for each group. Paired two-tailed t-test compared to WT, ΔUA2S-GlcNS **p = 0.004, and ΔUA-GlcNS6S-GlcA-GlcNS3S6S **p = 0.0057. ΔUA2S-GlcNAc6S not detected in WT and DKO SMGS. ΔUA-GlcNS6S-IdoA2S-GlcNS3S6S not detected in DKO SMGs. e Gene expression changes in HS biosynthetic enzymes in both male and female DKO SMGs normalized to the respective male or female WT SMGs and Rps29. Error bars are SM. n = 7 for WT-M and DKO-M SMGs. n = 3 for WT-F (Glce and Hs2st1) and DKO-F (Glce, Hs2st1, Ndst1, Ndst2 and Ndst3), n = 6 for WT-F (Ndst2, Ndst3) and DKO-F (Hs6st1), n = 10 for WT-F (Hs3st3b1 and Hs3st6), n = 11 DKO-F (Hs3st6), n = 14 DKO-F (Hs3st3a1 and Hs3st3b1), n = 15 WT-F (Ndst1), n = 16 WT-F (Hs3st1 and Hs3st3a1), n = 17 DKO-F (Hs3st1) and n = 18 DKO-F (Hs6st1). Unpaired two-tailed t-test compared to WT, ***p < 0.0001 for Hs3st3a1 and Hs3st3b1 in DKO-male and DKO-female. Source data are provided as a Source Data file.