Fig. 6.

Effects of Bergenin on NF-κB and STAT3 pathways in IL-1β-treated chondrocytes. (A) The top 20 pathways of co-targets. (B) Western blotting performed the activity of NF-κB, STAT3 and Jun signaling pathways in chondrocytes. (C) ImageJ software was used to quantify the density of western blotting bands shown in (B). (D) Immunofluorescence staining was used to determine nuclear translocation of p65 in chondrocytes. (E) The nuclear translocation of STAT3 was measured by immunofluorescence staining. Scale bar = 100 µm. The values are mean ± SD for 3 separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.