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. 2024 Sep 1;15:7608. doi: 10.1038/s41467-024-51962-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — a Stability of IPA1 protein with increasing levels of IPI7. Numbers indicate relative concentrations of A. tumefaciens carrying constructs used for co-infiltration. HA-GFP is an internal control for protein expression. Quantitation of protein bands of IPA1/GFP (b) and IPI7/GFP (c) for (a). Each value represents mean ± SD (n = 3 independent assays). d Stability of IPA1 with or without added IPI7 in rice protoplasts. The indicated plasmids were used to transfect rice protoplasts. IPA1-GFP protein amounts were quantitated by densitometry and normalized to the GFP-MYC level. e Statistical analysis of protein bands in (d) was conducted with three independent assays. Data are mean ± SD. IPA1 abundance in Nipponbare (NP), ipi7-ko plants (f), Ri22, and IPI7-OE plants (g). Samples were collected from indicated plants. IPA1 protein was probed in immunoblots with an IPA1 antibody and quantified by densitometry normalized to Heat Shock Protein 90 (HSP90). Similar results were obtained from three independent biological experiments in the dataset.