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. 2024 Sep 1;15:7608. doi: 10.1038/s41467-024-51962-x

Fig. 4. Ubiquitination of IPA1 mediated by IPI7 is essential for IPA1 to activate WRKY45 expression.

Fig. 4

a, b Effects of IPA1(S163D) and IPI7 on transactivation of WRKY45 promoter in tobacco leaves or rice protoplasts. a A. tumefaciens carrying plasmids were infiltrated into tobacco leaves. b Plasmids were used to transfect rice protoplasts. The luciferase (LUC) reporter is driven by the WRKY45 promoter (proWRKY45:LUC); Renilla LUC was used as the internal reference. D-luciferin was applied as the LUC substrate. Each value represents mean ± SD (n = 3 independent biological samples). Different letters indicate significant differences determined by the Tukey–Kramer test, p < 0.05 (one-way ANOVA was conducted, followed by a two-sided honestly significant difference (HSD) test for multiple comparisons). p values are shown in the Source Data file. c WRKY45 expression levels in NP and ipi7-ko plants were determined by RT-qPCR (n = 3 independent biological samples). ** indicates p < 0.01 (Two-tailed t-test for statistical analysis). Source data are provided as a Source Data file.