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. 2024 Sep 1;15:7608. doi: 10.1038/s41467-024-51962-x

Fig. 6. IPI7 contributes to IPA1-mediated disease resistance with lower fitness cost.

Fig. 6

a, b Effects of IPI7 knockout on IPA1(S163D)-mediated resistance to M. oryzae. Rice leaves were punch-inoculated with M. oryzae and incubated for a week. Lesion pictures are displayed, and 10 independent lesion lengths are measured (right panel) (a), M. oryzae populations determined based on fungal MoPot2 DNA content (b) are presented. Each value represents mean ± SD (n = 3 independent biological samples). c WRKY45 RNA levels in the leaves of NP, ipi7-ko, IPA1(S163D) over-expression (SD-OE) and SD-OE/ipi7-ko double transgenic plants were determined by RT-qPCR (n = 3 independent biological samples). d Main panicle morphology of NP, ipi7-ko1, SD-OE, and SD-OE/ipi7-ko plants. Scale bar = 5 cm. e Statistical analysis of main panicle length, primary and secondary branch numbers, and grains per main panicle of NP and different transgenic plants. Each value represents mean ± SD (n = 10 rice plants). Different letters indicate significant differences at p < 0.05 (one-way ANOVA was conducted, followed by two-sided HSD test for multiple comparisons). The corresponding p values can be found in the Source Data. Source data are provided as a Source Data file.