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. 2024 Sep 2;5(9):e716. doi: 10.1002/mco2.716

FIGURE 2.

FIGURE 2

Cytotoxicity of B‐cell activating factor receptor (BAFF‐R) chimeric antigen receptor (CAR) T cells on acute lymphocytic leukemia (ALL) cells directly confirms cytolysis of target cells. (A) Using a direct killing assay, BAFF‐R CAR T cells or non‐CAR T cells were co‐cultured with either green fluorescent protein (GFP)‐labeled tumor cells (Nalm‐6 WT or BAFF‐R‐knockout [KO] cells) at 20:1 (E:T ratio) for 24 h. The live GFP‐expressing tumor cells were quantified with a gating strategy that gated on the live cells first and then the GFP‐positive target cells. These dot plots are representative of data obtained from three independent experiments. (B) By setting the non‐CAR T cells at 100% for each of three experiments, the non‐CAR T group served as a baseline to normalize the percentile of live tumor cells in BAFF‐R CAR T group. The results were showed as mean ± SEM from four replicates and representative from three independent experiments (∗∗∗ p < 0.001).