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. 2024 Sep 2;5(9):e716. doi: 10.1002/mco2.716

FIGURE 4.

FIGURE 4

Cytotoxicity of B‐cell activating factor receptor (BAFF‐R) chimeric antigen receptor (CAR) T cells against CD19‐knockout (KO) MEC‐1 cell line. (A) BAFF‐R or CD19 expression in MEC‐1 WT or MEC‐1 CD19‐KO were characterized by BAFF‐R‐AF647 or CD19 APC antibodies staining and analyzed with flow cytometry. (B and C) BAFF‐R or CD19 CAR T cells were incubated with MEC‐1 WT or MEC‐1 CD19‐KO at an E:T ratio of 2:1, respectively. Cell surface expression of CD107a was analyzed on gated CD8+ CAR T cells (B) or CD4+ CAR T cells (C); non‐CAR T cells were used as controls. (D) BAFF‐R or CD19 CAR T cells were co‐cultured with either MEC‐1 WT or MEC‐1 CD19‐KO cell lines at an E:T ratio of 4:1. IFN‐γ was assessed from the harvested supernatant after 72 h. Non‐CAR T cells were used as control. The results were showed as mean ± SEM from four replicates and representative from three independent experiments (∗∗∗ p  < 0.001; ns, no significant differences).