FIGURE 5.

Immunophenotyping of isolated primary B tumor cells from chronic lymphocytic leukemia (CLL) patients. (A) Primary B tumor cells from CLL patients were isolated from peripheral blood mononuclear cells (PBMCs) to serve as target cells in chimeric antigen receptor (CAR) T‐cell function assays. Enriching B cells significantly reduces the proportion of T cells in the target cell population. (B) B‐cell activating factor receptor (BAFF‐R) expression in the enriched B cells from the three CLL patients was characterized using BAFF‐R‐AF647 antibodies, followed by flow cytometry analysis. Isotype antibody served as staining controls.