FIGURE 6.

Cytotoxicity of B‐cell activating factor receptor (BAFF‐R) chimeric antigen receptor (CAR) T cells from healthy donors against primary B tumor cells from chronic lymphocytic leukemia (CLL) patients. The BAFF‐R CAR T cells from healthy donors were produced and then incubated with primary B tumor cells from three CLL patients at 2:1 ratio, followed by a CD107a assay. Used MEC‐1 WT cells as a positive control. The analysis focused on CD8⁺ or CD4⁺ CAR T cells, with non‐CAR T cells as negative controls. Cytotoxicity of BAFF‐R CAR T cells derived from healthy donor A against primary B tumor cells from three CLL patients was observed: CLL patient 1 (CD8⁺ in A; CD4⁺ in B); CLL patient 2 (CD8⁺ in C; CD4⁺ in D); and CLL patient 3 (CD8⁺ in E; CD4⁺ in F).