FIGURE 8.

Chronic lymphocytic leukemia (CLL) patient‐derived B‐cell activating factor receptor (BAFF‐R) chimeric antigen receptor (CAR) T elicited ex vivo cytotoxicity against autologous B cells. Using a CD107a degranulation assay, the cytotoxic efficacy of CLL patient‐derived BAFF‐R CAR T cells was assessed against a collection of previously established target cells: Nalm‐6 WT, BAFF‐R‐KO Nalm‐6, and MEC‐1 WT cells. Most significantly, the cytotoxic efficacy of CLL patient‐derived BAFF‐R CAR T cells showed cytotoxicity against autologous B tumor cells. Non‐CAR T cells from the same patient were used as a control. Cytotoxicity analysis of was performed on the CD8⁺ CAR T‐cell populations from CLL patient 1 (A), CLL patient 2 (B), and CLL patient 3 (C). Note that the cytotoxicity was also gated on the CD4⁺ CAR T‐cell population and included in Figure S2A‒C.