GGCT knockdown repressed the formation of xenograft tumors via REST. (A) Representative images of each group in vivo xenograft tumors (n = 5). (B) Representative images of tumor harvested from nude mice inoculated with T98G cells of Control group, GGCT shRNA + REST OV group, GGCT shRNA group. (C) Tumor volume was measured every 3 days (n = 5). (D) Ki-67, GGCT and REST protein expression was detected by IHC staining in tumor tissues of Control group, GGCT shRNA + REST OV group, GGCT shRNA group. (E) Ki-67, (F) GGCT, (G) REST protein expression was statistical analyzed from IHC staining results (n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001.