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. 2024 Aug 10;49:102083. doi: 10.1016/j.tranon.2024.102083

Fig. 8.

Fig. 8

REST increased GGCT expression via inhibiting miR-34a-5p expression in glioma cells. (A) Veen diagram was used to visualize the common miRNAs in DE-miRNAs and miRNAs of target GGCT 3′UTR. (B) RT-qPCR assay was used to confirm the miRNAs of REST negatively regulate in T98G cells. (C) has-miR-34a-5p binds to the site of GGCT 3′UTR. (D) Dual-luciferase reporter genes activity assay in T98G cells. (E) RT-qPCR assay was used to detect the GGCT and miR-34a-5p mRNA expression in T98G cells. (F) Western blot assay was used to detect the GGCT protein expression. (G) GGCT protein expression level was statistic in T98 an dU251 cells. (H) Schematics diagram depicting the key findings of this study. * P < 0.05, ** P < 0.01, *** P < 0.001.