Figure 1.
Effect of different concentrations of RSG on chondrocytes
(A) Structural formula of RSG (molecular formula: C18H19N3O3S; molecular weight: 357.43).
(B and C) Chondrocytes were treated with varying concentrations of RSG (0, 12.5, 25, 50, 100, 200 μM) for 24 and 48 h (n = 6 per group).
(D) Levels of ACSL4 in chondrocytes after stimulation with different concentrations of RSG for 24 h were measured by Western Blot.
(E) Variation in ACSL4 mRNA expression levels with different concentrations of RSG (n = 4 per group).
(F) Protein expression levels of ACSL4 in chondrocytes treated with varying concentrations of RSG (n = 3 per group).
(G) Chondrocyte morphology revealed by toluidine blue staining (scale bar = 100 μm). Two-tailed p values were calculated, and statistical significance was defined as p < 0.05, denoted as 'ns' for no statistical difference, and indicated as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. All experiments and images shown are representative.