Figure 5.

RSG reduces the levels of lipid peroxidation within chondrocytes initiated by IL-1β/FAC

(A) Lipid peroxides in chondrocytes under different treatments detected by Liperfluo probes (scale bar = 200 μm).

(B) Quantification of Liperfluo fluorescence (n = 3 per group).

(C) The MDA contents were determined by the lipid peroxidation MDA assay kit (n = 4 per group).

(D) Detection of lipid peroxidation by C11 BODIPY^581/591 fluorescent probe (scale bar = 200 μm).

(E) Quantification of C11 BODIPY^581/591 fluorescence (n = 3 per group).

(F) 4-HNE levels in the chondrocytes determined by ELISA (n = 4 per group).

(G) Fluorescence intensity of Liperfluo detected using flow cytometry.

(H) Quantitative analysis of flow cytometric measurements (n = 3 per group).

(I) Fluorescence intensity of C11 BODIPY^581/591 detected using flow cytometry.

(J) Quantitative analysis of flow cytometric measurements (n = 3 per group). Two-tailed p values were calculated, and statistical significance was defined as p < 0.05, denoted as 'ns' for no statistical difference, and indicated as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. All experiments and images shown are representative.