Figure 6.

RSG alleviates IL-1β/FAC-induced injury to mitochondria and lysosomes

(A) Mitochondria in chondrocytes were visualized by transmission electron microscopy, with red arrows indicating the location of mitochondria (scale bar = 500 nm).

(B) Detection of the changes in mitochondrial membrane potential using Rhodamine 123 (scale bar = 200 μm).

(C) Fluorescence intensity of Rhodamine 123 detected using flow cytometry.

(D) Quantitative analysis of flow cytometric measurements (n = 3 per group).

(E) Lysosomal and mitochondrial mass were evaluated by LysoTracker red and Mito-Tracker Green staining respectively (scale bar = 200 μm).

(F) Fluorescence intensity of LysoTracker red detected using flow cytometry.

(G) Quantitative analysis of flow cytometric measurements (n = 3 per group).

(H) Fluorescence intensity of Mito-Tracker Green detected using flow cytometry.

(I) Quantitative analysis of flow cytometric measurements (n = 3 per group). Two-tailed p values were calculated, and statistical significance was defined as p < 0.05, denoted as 'ns' for no statistical difference, and indicated as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. All experiments and images shown are representative.