Figure 7.

RSG preconditioning confers anti-ferroptotic property upon chondrocytes

(A–C) Immunofluorescence staining of ACSL4, GPx4, and TfR1 (scale bar = 200 μm).

(D) Prussian blue staining was performed on chondrocytes, with red arrows indicating iron deposition (scale bar = 200 μm).

(E–G) Quantification of immunofluorescence staining for ACSL4, GPx4, and TfR1 (n = 3 per group). Two-tailed p values were calculated, and statistical significance was defined as p < 0.05, denoted as 'ns' for no statistical difference, and indicated as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. All experiments and images shown are representative.