Figure 9.

RSG alleviated chondrocyte inflammatory responses, ECM degradation, and ferroptosis within the surgery-induced mouse OA model

(A) IHC assay of 4-HNE, ACSL4, ADAMTS5, COX-2, TfR1, GPx4, MMP3, and Nrf2 in articular cartilage of each group (scale bar = 100 μm).

(B–I) Quantification of IHC analysis of 4-HNE, ACSL4, ADAMTS5, COX-2, TfR1, GPx4, MMP3, and Nrf2 (n = 8 per group). Two-tailed p values were calculated, and statistical significance was defined as p < 0.05, denoted as 'ns' for no statistical difference, and indicated as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. All experiments and images shown are representative.