REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
MMP3	FineTest, Wuhan, China	FNab05244
ADAMTS5	Bioss, Beijing, China	bs-3573R
GLUT1	ABclonal, Wuhan, China	A6982
MMP13	FineTest, Wuhan, China	FNab05235
CHAC1	ABclonal, Wuhan, China	A15584
TfR1	FineTest, Wuhan, China	FNab08931
ACSL4	ABclonal, Wuhan, China	A20414
RGS4	FineTest, Wuhan, China	FNab07271
SLC7A11	FineTest, Wuhan, China	FNab10533
LPCAT3	ABclonal, Wuhan, China	A17604
GPx4	FineTest, Wuhan, China	FNab03622
COX-2	FineTest, Wuhan, China	FNab10407
4-HNE	Bioss, Beijing, China	bs-6313R
Nrf2	Servicebio, Wuhan, China	GB113808-100
Vinculin	FineTest, Wuhan, China	FNab09799
GAPDH	Cell Signaling Technology, Massachusetts, USA	5174S
FITC-conjugated Goat anti-Rabbit IgG (H + L)	ABclonal, Wuhan, China	AS011
Anti-rabbit IgG (H + L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)	Cell Signaling Technology, Massachusetts, USA	4413S
HRP Goat Anti-Rabbit IgG (H + L)	ABclonal, Wuhan, China	AS014
Chemicals, peptides, and recombinant proteins
Complete DMEM/F12 Medium	Procell, Wuhan, China	PM150310B
0.25% trypsin-EDTA	Servicebio, Wuhan, China	G4001
GP-transfect-Mate	GenePharma, Shanghai, China	G04009
Tissue Lysis Buffer for Glucose Assay	Beyotime, Shanghai, China	S3062
Cell Lysis Buffer	Beyotime, Shanghai, China	P0013
RIPA Lysis Buffer	Servicebio, Wuhan, China	G2033
PMSF	Servicebio, Wuhan, China	G2008
TBST Buffer	Servicebio, Wuhan, China	G2150
Super-sensitive ECL Chemiluminescent Substrate	Biosharp, Hefei, China	BL520B
Western Blot Running Buffer	Servicebio, Wuhan, China	G2144
Western Blot Transfer Buffer	Servicebio, Wuhan, China	G2145
10% SDS-PAGE	Biotides, Bejing, China	WB1102
4% Paraformaldehyde Fixative Tissue Fixation Fluid	Servicebio, Wuhan, China	G1101
4% Paraformaldehyde	Beyotime, Shanghai, China	P0099
Toluidine Blue Stain Solution for Cell	Solarbio, Beijing, China	G3660
Rosiglitazone	Rhawn, Shanghai, China	R014317
Erastin	Macklin, Shanghai, China	E872563
PBS buffer	Procell, Wuhan, China	PB180327
Ferric Ammonium Citrate	Rhawn, Shanghai, China	R017760
Recombinant Mouse Interleukin-1 beta	PeproTech, New Jersey, USA	211-11B
Iron Dextran	Macklin, Shanghai, China	I812123
TB Green® Premix DimerEraser™	Takara, Kyoto, Japan	RR091A
FerroOrange	Dojindo, Kumamoto, Japan	F374
Mito-FerroGreen	Dojindo, Kumamoto, Japan	M489
Lipid Peroxidation Probe-BDP 581/591 C11	Dojindo, Kumamoto, Japan	L267
Liperfluo	Dojindo, Kumamoto, Japan	L248
mtSOX Deep Red	Dojindo, Kumamoto, Japan	MT14
Mito-Tracker Green	Beyotime, Shanghai, China	C1048
LysoTracker red	Beyotime, Shanghai, China	C1046
ER-Tracker Green	Beyotime, Shanghai, China	C1042S
Golgi-Tracker Red	Beyotime, Shanghai, China	C1043
Toluidine Blue Cartilage Stain Solution	Servicebio, Wuhan, China	G1032
Saffron-O and Fast Green Stain Solution	Servicebio, Wuhan, China	G1053
Hematoxylin-Eosin Stain Solution	Servicebio, Wuhan, China	G1005
Isoflurane	RWD Life Science	R510-22
DAB Stain Solution	Servicebio, Wuhan, China	G1212
Dimethyl sulfoxide	Solarbio, Beijing, China	D8371
Critical commercial assays
Glucose assay kit with O-toluidine	Beyotime, Shanghai, China	S0201S
Bicinchoninic Acid Protein Assay Kit	KeyGEN BioTECH, Nanjing, China	KGB2101
Cell Counting Kit-8	Beyotime, Shanghai, China	C0037
Total RNA Isolation Kit	Beibei Biotechnology, Zhengzhou, China	082001
PrimeScript™ RT Reagent Kit with gDNA Eraser	Takara, Kyoto, Japan	RR047A
Annexin V-FITC/Propidium Iodide Apoptosis Detection Kit	Dojindo, Kumamoto, Japan	AD10
Calcein-AM/PI Double Staining Kit	Dojindo, Kumamoto, Japan	C542
BeyoClick™ EdU Cell Proliferation Kit with Alexa Fluor 555	Beyotime, Shanghai, China	C0075S
Senescence-Associated β-Galactosidase Staining Kit	Solarbio, Beijing, China	G1580
Prussian Blue Iron Stain Kit for Cell	Solarbio, Beijing, China	G1426
Reactive Oxygen Species Assay Kit	Beyotime, Shanghai, China	S0033S
GSH and GSSG Assay Kit	Beyotime, Shanghai, China	S0053
Malondialdehyde Assay Kit	Beyotime, Shanghai, China	S0131S
Ferrous Ion Content Assay Kit	Solarbio, Beijing, China	BC5410
Glutamate Content Assay Kit	Solarbio, Beijing, China	BC1585
Glutamine Content Assay Kit	Geruisi, Soochow, China	G0429W
Superoxide Dismutase Activity Assay Kit	Solarbio, Beijing, China	BC5165
Mitochondrial Membrane Potential Assay Kit with JC-1	Beyotime, Shanghai, China	C2006
Mitochondrial Membrane Potential Assay Kit with Rhodamine 123	Beyotime, Shanghai, China	C2008S
Ultra-Sensitive TM SP (Mouse/Rabbit) IHC Kit	MXB Biotechnologies, Fuzhou, China	KIT-9710
Pepsin Antigen Repair Solution	Servicebio, Wuhan, China	G0142
Mouse 4-Hydroxynonenal ELISA Kit	FineTest, Wuhan, China	EM1583
Mouse Nitric Oxide Synthase 2 (iNOS) ELISA Kit	FineTest, Wuhan, China	EM0272
Mouse Phospholipid Hydroperoxide Glutathione peroxidase (GPx4) ELISA Kit	FineTest, Wuhan, China	EM1964
Mouse Interleukin-6 (IL-6) ELISA Kit	ABclonal, Wuhan, China	RK00008
Mouse Cyclooxygenase-2 (COX-2) ELISA Kit	ABclonal, Wuhan, China	RK03142
Mouse Tumor Necrosis Factor-alpha (TNF-α) ELISA Kit	ABclonal, Wuhan, China	RK00027
Experimental models: Cell lines
Primary mouse chondrocytes	Procell, Wuhan, China	CP-M087
Experimental models: Organisms/strains
C57BL/6 mice	Guangdong Medical Laboratory Animal Center	Not Applicable
Oligonucleotides
Primer for qPCR	Table S1	Not Applicable
Software and algorithms
ImageJ/Fiji 2.9.0	National Institutes of Health	https://imagej.net/ij/
GraphPad Prism 9.5	GraphPad Software, San Diego, USA	https://www.graphpad.com/
G∗Power 3.1.9.7	Universität Düsseldorf, Germany	https://www.psychologie.hhu.de/arbeitsgruppen/allgemeine-psychologie-und-arbeitspsychologie/gpower