Table 1.

The used concentration of CHIR99021 and IWP-2 chemicals and measurements of regenerated digit tips following treatment of digit tips

Target gene	Primer sequence	Accession number	Annealing temperature	Product size
Tcf 1	F: 5' TACCCTACAAATGCTTCTCCTG 3' R: 5' AAACGTATCCTAGTCCCTCCT 3'	NM_009327.3	60 ⁰C	247 bp
β-catenin	F: 5' AGAACACTAATTCATAATCACGCT 3' R: 5'GGCTCAAATAACACCTCTTACTG 3'	NM_001165902.1	60 ⁰C	209 bp
GAPDH	F: 5’ GACTTCAACAGCAACTCCCAC 3’ R: 5’ TCCACCACCCTGTTGCTGTA 3’	NM_008084	60 ⁰C	125 bp