Table 1.

Primer sequences used for RT-PCR quantification of IL-6, IL-1β, TNF-α and Keap1, Nrf2, HO-1, NQO-1

Gene	Primer Pair (5’-3’) F，forward; R，reverse
Rat -β-Actin	F 5’-GTAAAGACCTCTATGCCAACA-3’ R 5’-GGACTCATCGTACTCCTGCT-3’
Rat -IL-6	F 5’- CCAGAGATACAAAGAAATGATGG 3’ R 5’- ACTCCAGAAGACCAGAGGAAA - 3’
Rat -IL-1β	F 5’- CTCCATGAGCTTTGTACAAGG -3’ R 5’- TGCTGAT GTACCAGTTGGGG -3’
Rat -TNF-α	F 5’- CGGGGTGATCGGTCCCCAAAG -3’ R 5’- GGAGGGCGTTGGCGCGCTGG -3’
Rat -Keap1	F 5’-CACCAGGGCAGGATCTAC-3’ R 5’-TTGCTTCCGACAGGGTTC-3’
Rat -Nrf2	F 5’-CTGCTGCCATTAGTCAGTCG-3’ R 5’-GCCTTCAGTGTGCTTCTGGT-3’
Rat -HO-1	F 5’-CAGAGTTTCTTCGCCAGAGG-3’ R 5’-TGAGTGTGAGGACCCATCG-3’
Rat -NQO-1	F 5’-TCCAGAAACGACATCACAGG-3’ R 5’-AGCTACAATATCCGGGCTCA-3’

Primers used are all synthesized by Shanghai Bioengineering Co., Ltd