. 2024 Aug 23;25(8):308. doi: 10.31083/j.rcm2508308

Table 1.

Primer and probe sequences for LPA-KIV2 copy number identification.

Target	Primer and probe sequences
LPA-KIV2 exon4	Forward	${{}^{\prime}}$ -GTCAGGTGGGAGTACTGCAA- ${{}^{\prime}}$
	Reverse	${{}^{\prime}}$ -CGACGGCAGTCCCTTCTG- ${{}^{\prime}}$
	Probe	${{}^{\prime}}$ -ROX-CCTGACGCAATGCTCA-BHQ2- ${{}^{\prime}}$
LPA-KIV2 exon5	Forward	${{}^{\prime}}$ -GCACATACTCCA CCACTGTCA- ${{}^{\prime}}$
	Reverse	${{}^{\prime}}$ -GCGAGTGTGGTGTCATAGATGA- ${{}^{\prime}}$
	Probe	${{}^{\prime}}$ -6FAM-CTTGGCAGGTTCTTCC- ${{}^{\prime}}$
RNAse P	Forward	${{}^{\prime}}$ -AGATTTGGACCTGCGAGCG- ${{}^{\prime}}$
	Reverse	${{}^{\prime}}$ -GAGCGGCTGTCTCCACAAGT- ${{}^{\prime}}$
	Probe	${{}^{\prime}}$ -CY5-TTCTGACCTGAAGGCTCTGCGCG-BHQ2- ${{}^{\prime}}$

Abbreviations: LPA-KIV2 exon4 and exon5, primer and probe sequences to evaluate kringle IV type 2 repeats in exons 4 and 5 of the LPA gene; RNAse P, ribonuclease type P.