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. 2024 Jul 30;103(10):104146. doi: 10.1016/j.psj.2024.104146

Figure 1.

Figure 1

Interaction of GAstV-2 VP70 with VIM. (A) Coomassie Brilliant Blue staining after SDS–PAGE electrophoresis. GST-VP70 fusion protein served as the bait protein, while the GEK cell lysate was used as the target protein library for GST pull-down analysis. (B) MALDI–TOF MS spectra of peptides of VIM. The distinct bands were carefully excised and sent to Sangon Biotech for MALDI-TOF MS assay, revealing VIM. (C) Immunofluorescence staining of VIM in LMH and GEK cells (scale bars: 15 μm). (D and E) Co-IP and GST pull-down assays of VP70 and VIM using both forward and reverse pull-down approaches. These results indicate a direct interaction between the VP70 protein and VIM.