Figure 3. Intertumoral and intratumoral heterogeneity of NKG2C expression in three patients with GBM. (A) Representative high-magnification image showing the co-expression of SOX2 (magenta), SOX4 (cyan), and NKG2C (green) in tumor cells. The upper panels display individual DAPI/NKG2C, DAPI/SOX2, and DAPI/SOX4 staining. (B–D) Representative images of the immunofluorescence analysis of the expression of SOX2 (magenta), SOX4 (cyan), and NKG2C (green) in the periphery (top images) and tumor core (bottom images) of three different patients with GBM. Nuclei were counterstained with DAPI (blue). The left images show the overall tissue structure with boxes indicating regions of interest magnified on the right. Scale: 10 µm (A) 100 µm for the main images and 50 µm for the insets (B–D). DAPI, 4',6-diamidino-2-phenylindole; GBM, glioblastoma; qRT-PCR, quantitative real time-polymerase chain reaction; UMAP, uniform manifold approximation and projection.