Figure 7.

Comparative analysis of in vitro cytotoxicity of CD33^PAN CAR T cells

(A) CD8⁺ or (B) CD4⁺ CD33^PAN (1H7, 9G2, and 3A5) CAR T cells were co-cultured with ML-1 cells with CRISPR-Cas9-mediated deletion of the endogenous CD33 locus (CD33^KO) or parental CD33⁺ AML cells and assessed for cytotoxicity by flow cytometry after overnight co-culture. Percent cell death was calculated by subtracting percent cell death from background cell death in non-T cell-containing wells. (C) Surface tCD19 expression as measured by Quantibrite-PE. Shown are mean ± SEM from technical triplicates of one representative out of two separate experiments. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns (not significant) by two-way ANOVA with post hoc Tukey correction.