Figure 2.

Heatmap summarizing preferential binding of human galectin (Gal)-1, -3, -4, -7, and -12 by competitive solid phase assays (SPA) using immobilized asialofetuin. Competitive interactions of galectins with a panel of 21 oligosaccharides, colored according to their IC₅₀ values. Best binders are colored in white and low binders in blue. Glycans with IC₅₀ values higher than 25 mM (poor ligands) are shown in black. The oligosaccharides selected for ITC analysis are marked in bold and italics. ITC, isothermal titration calorimetry.