Table 1.
ITC evaluation of thermodynamic binding parameters for human Gal-1, -3, -4, -7, and -12 toward the best three ligands previously identified by SPA
| Galectin | Oligosaccharide | Kd (μM) | ΔH (kJ/mol) | -TΔS (kJ/mol) |
|---|---|---|---|---|
| Gal-1 | LNnT | 72 ± 8 | −9.0 ± 0.9 | −14.7 ± 0.8 |
| LNnH | 69 ± 7 | −11.8 ± 1 | −11.9 ± 0.8 | |
| LNnO | 73 ± 7 | −12.7 ± 2 | −10.9 ± 0.0 | |
| Gal-3 | LNnT | 95 ± 9 | ND∗ | ND∗ |
| LNnH | 42 ± 5 | −6.2 ± 0.6 | −19 ± 2 | |
| LNnO | 40 ± 3 | −4.6 ± 0.4 | −21 ± 2 | |
| Gal-4 | 2′-FL | 130 ± 9 | ND∗ | ND∗ |
| BGA2 | 65 ± 5 | −25 ± 2 | 1.1 ± 0.2 | |
| BGA6 | 51 ± 3 | −32 ± 3 | 7.4 ± 0.6 | |
| Gal-7 | LNT | 106 ± 8 | ND∗ | ND∗ |
| 3′-SL | 97 ± 9 | ND∗ | ND∗ | |
| BGA2 | 103 ± 8 | ND∗ | ND∗ | |
| Gal-12 | 3-FL | 56 ± 5 | −66 ± 5 | 41 ± 3 |
| LeX | 80 ± 9 | ND∗ | ND∗ | |
| SFL | 151 ± 11 | ND∗ | ND∗ |
No good fit of the thermodynamic parameters could be obtained for binding affinities above 80 μM (ND∗, not determined).