Fig. 1.

GLO1 is Attenuated in Skeletal Muscle from Individuals with Obesity

Human skeletal muscle biopsy samples (vastus lateralis) were analyzed for A) GLO1 protein abundance, B) GLO1 activity, and C) GLO1 transcripts. A representative Western blot for GLO1 and total protein used for normalizing signal quantification of target proteins are displayed in panel D. Protein level of GLO1's transcription factor NRF2 (E), its negative regulator KEAP1 (F), and their ratio (G) were measured via Western blot. A representative Western blot for NRF2, KEAP1, and total protein are displayed in panel H. Data are presented as mean ± SEM with individual data points superimposed. Data were compared via independent student's T Test or Mann-Whitney U test, depending on normality, with significance set to p < 0.05.