Fig. 11.

Proteomic Consequences of GLO1 Knock Down in Human Immortalized Myotubes.

To explore the proteomic consequences of decreased GLO1 abundance in human immortalized myotubes, we knocked down GLO1 by treating myotubes with GLO1 siRNA which resulted in an approximately 50 % reduction in GLO1 protein as confirmed by Western blotting (B–C) and quantification via TMT mass spec (C). PCA demonstrates the unique clustering of samples in the scRNA and siRNA-treated samples and significantly altered proteins are visually demonstrated in the volcano plot in panel E. F) Hierarchical clustering of a heat map of significantly changed proteins with siRNA identified several unique families of similarly altered proteins within the siRNA-treated group. This is confirmed and visually represented in the dot plot in G) demonstrating that many pathways involving muscle contraction and organization are attenuated in human myotubes with attenuated GLO1 protein, exemplifying the importance of GLO1 in the muscle cell.