Fig. 5.

Effect of SIRT2 Knock Down In Human Immortalized Myotubes on GLO1 Acetylation

Lysine-acetylated proteins (AcK) were immunoprecipitated from Human Immortalized Myotubes transfected with SIRT2 siRNA for 48 h and subsequently immunoblotted (IB) for GLO1 (A,B), and P53 (A,C). Signal quantification of acetylated GLO1 (B), and P53 (C) were made relative to the signal of acetylated proteins that were immunoprecipitated to account for any technical variance in the IP procedure. Data are presented as mean ± SEM with individual data points superimposed. Data were compared via independent student's T Test with significance set to p < 0.05.