Fig. 8.

Effect of NAMPT Knock Down and NR Rescue On GLO1 Transcripts in Human Immortalized Myotubes

Human Immortalized Myotubes transfected with NAMPT siRNA for 48 h, or siRNA for 48 h with 0.5 mM nicotinamide riboside (NR) treatment for the final 24 h (NAMPT siRNA + 0.5 mM NR). NRF2 (A), KEAP1 (B), SIRT2 (C,D), and SIRT 1 (F) proteins were unaltered by NAMPT knockdown and NR. Representative western blots for protein targets are provided in panels E and G. GLO1 transcripts were attenuated by NAMPT siRNA and rescued by NR (H). Copies of GLO1's transcription factor NRF2 and its negative regulator KEAP1 were not affected by NAMPT siRNA but NRF2 copies were enhanced by co-treatment with NR (I–J). Data are presented as mean ± SEM with individual data points superimposed. Data were compared via One-way ANOVA using Bonferroni Post Hoc analysis with significance set to p < 0.05.