Fig. 9.

Effect of NAMPT Knock Down and NR Rescue In Human Immortalized Myotubes on GLO1 Acetylation

Lysine-acetylated proteins (AcK) were immunoprecipitated from Human Immortalized Myotubes transfected with NAMPT siRNA for 48 h, or siRNA for 48 h with 0.5 mM nicotinamide riboside (NR) treatment for the final 24 h (NAMPT siRNA + 0.5 mM NR) and subsequently immunoblotted (IB) for GLO1 (A,C), and P53 (B,D). Signal quantification of acetylated GLO1 (B), and P53 (C) were made relative to the signal of acetylated proteins that were immunoprecipitated to account for any technical variance in the IP procedure. Data are presented as mean ± SEM with individual data points superimposed. Data were compared one-way ANOVA using Bonferroni Post Hoc analysis with significance set to p < 0.05.