Fig. 1.

Experimental design used to generate the proteomic data. A. Key steps for isolating T cell populations B. Gating strategy used for fluorescence-activated cell sorting (FACS) to obtain MAIT cells and non-MAIT T cell populations at a high purity. Gating strategy is shown for cells pre and post-FACS. C. Key steps followed for the obtention of trypsin digested peptide samples and proteomic data acquisition.